The Ultimate Guide to Mushroom Grow Bags for Fruiting and Spawn (2024)

What is a Mushroom Grow Bag?

A mushroom grow bag is, quite simply, a bag designed for growing mushrooms[i]. Many kinds of mushrooms can be grown in a bag, depending on what kind of substrate is put in the bag. There are several brands and types, but all good grow bags have several features in common.

Polypropylene

Grow bags are made of polypropylene plastic designed to withstand high temperature. The plastic is durable, flexible, clear enough to make monitoring growth easy, and relatively low-cost. There are bags being developed using “greener” materials, but none of them have come to market yet.

Autoclavable

The reason temperature resistance is important is that both the substrate and the inside of the bag must be sterilized before inoculation. A convenient sterilization method is to stick the bag and its contents in an autoclave—except that most plastic bags would melt in such conditions. “Autoclavable” just means that the bag can be safely used in an autoclave. An autoclave is a machine that uses steam under pressure to kill bacteria.

Filter Patch

A filter patch is a small area of the bag that allows air in through an extremely fine mesh. The mesh is so fine that airborne bacteria and the spores of unwanted fungal species (weeds) can’t get through. Unlike a vegetable garden, a mushroom grow set-up can’t be weeded; the weeds have to be kept out from the beginning or the batch must be abandoned. Mushroom grow bags needs filter patches because while it is important to keep out weeds, the mycelium must be able to breathe (another thing, besides the need for food, that fungi have in common with animals).

Gusseted

A gusseted bag has folds in the sides so that a large, very roomy bag can still fold nice and flat when not in use. The mushrooms don’t care whether grow bags are gusseted, but the feature makes things easier for growers be organized.

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Why Use Mushroom Grow bags?

It’s possible to grow mushrooms without bags. Of course, some substrates, such as logs, don’t need and can’t fit in bags, but even substrates often used with bags, such as coffee grounds or sawdust, don’t actually require them. Alternatives include wide-mouth glass jars and plastic buckets and tubs (these are sometimes available used from restaurants at no cost), provided the containers in question can be sterilized and fitted with air filters[ii]. There are also low-temperature sterilization methods and ways to grow mushrooms that don’t require sterilization at all—such methods dramatically broaden the range of set-ups that can be used.

Some growers seek to avoid grow bags specifically to avoid creating so much plastic waste, since the bags are generally single-use. There may be other reasons for avoiding grow-bags, too.

However, grow bags offer certain features that are difficult to access otherwise:

  • It’s easy to cut holes in a bag, allowing the fungus to fruit out in all directions, instead of just upwards. The same can’t be said of glass.
  • The clear plastic makes it easy to monitor the mycelium as it colonizes the substrate. With buckets, it’s harder to tell when colonization is complete and when the fungus is ready to fruit.
  • The spent substrate—or the fully colonized spawn, in the case of spawn bags—is easy to remove from a bag. Because the mycelium tends to hold the substrate together in a clump, getting the clump out of a jar or even a bucket can be a real challenge.
  • The flexible bag makes it easy to mix the spawn thoroughly through the substrate; just squeeze. The same can’t be said of a hard-sized container.
  • Start-up costs are low, even for large operations, since bags are cheap.

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What Are Mushroom Grow Bags Used For?

Mushroom grow bags are used for growing mushrooms, that much is clear, but there are multiple stages to the mushroom-growing process, and grow bags can be used at several of them.

Spawn Bags

“Spawn” is material growers use to multiply up the amount of mycelium they have quickly, so as to be able to grow a much larger crop[iii]. Rye and other grains are popular spawn media, though there are others. Generally speaking, the procedure begins with the germination of spores on a Petri dish of nutrients, where a small, baby mycelium begins to grow. The baby in the dish is then transferred to a larger quantity (up to several pounds) of sterilized spawn substrate. Once the spawn is fully colonized, it can be used to inoculate the substrate used for growth and fruiting—the spawn can also be used to inoculate more spawn. Many people buy bags of spawn ready to go, but some growers produce their own.

Because spawn needs sterile conditions and must be monitored closely, clear polypropylene bags are a good option.

Fruiting Blocks

A fruiting block is a unit of some mushroom substrate that does not naturally hang together, such as sawdust. The grow bag serves both to neatly contain the material and to keep out weed spores and other contaminants. Once the mycelium has fully colonized the block, weeds can no longer gain a foot-hold and the container can be opened to allow the fungus enough room and air to fruit. Again, the clear bag makes it easy to see whether colonization is complete, and the plastic can be cut open to allow more fruiting without risking damage to the mycelium below.

Fruiting in the Bag

It is also possible to keep the bag closed during fruiting, though in this case the bag must be only partially filled to give the mushrooms plenty of room to grow. The bag then serves as a miniature fruiting room where humidity and other factors are easy to control. The trick is to open the bag frequently; the air filter alone doesn’t allow enough air, and the mature mycelium, like an animal in a similar situation, would gradually run out of oxygen if kept too enclosed.

Allowing the fungus to fruit entirely inside the bag means not needing a climate-controlled room. For some species, there is an added benefit. Even with opening the box often, there will be less oxygen and more carbon dioxide, in a bag than out of it, and the difference can sometimes force mushrooms into startlingly different growth forms.

Choosing the Right Mushroom Grow Bag

Not all mushroom grow bags are exactly the same, but choosing the right bags for a given operation means paying attention to just three variables: filter size, bag size, and bag thickness.

Filter Size

Filter sizes vary. That is, the size of the holes, or pores, in the filter varies, usually from 0.2 micron to 5 microns. The smaller the pore size, the less can get through. That means fewer spores and other contaminants, but it also means less air. The key is to strike workable a balance between the need for air and the need for protection. For most purposes, the filter should be no larger than 0.5 micron.

Bag Size

There is no hard and fast rule about bag size. Bags that are too small are inefficient; bags that are too big are inconvenient—but what sizes are too big or too small varies from grower to grower. Points to consider include the size of the sterilizer and the size of the hoped-for crop. Above all, how big will a bag of a given size be when full?

A large bag, one that is eight by five inches across and 18 inches tall, can hold about five pounds of inoculated substrate.

Thickness

The thicker the plastic, the stronger the bag—but the stronger bags are also more expensive. The normal range is between 2.2 mil to 4.0 mil. Growers can start off with bags at the thin and inexpensive end of the scale, and if the bags rip too often (a ripped bag is vulnerable to contamination), step up to something thicker. The occasional rip is not considered a problem, just part of the cost of doing business.

How to Seal a Mushroom Grow Bag

Grow bags must be sealed after being inoculated and the bags themselves don’t come with seals. Wire ties and zip ties both work, but the time necessary to apply these ties, though brief on a per-bag basis, adds up for people who have to seal a lot of bags. Large-scale growers buy impulse sealers and find the greater expense is worth it.

Can I re-use a Mushroom Grow Bag?

Yes, mushroom grow bags are reusable, but not necessarily as mushroom grow bags. The plastic gets weaker with use, and many growers do not want to risk a bag coming apart—or developing tiny holes through which weed spores could pass—mid-cycle. But the bags can be reused in other contexts. For example, material that needs to be sterilized and then transferred to some other container can be boiled inside an old grow bag. Bags can also be used for making straw logs. Some growers are even experimenting with processing the material into mushroom substrate; there are fungi capable of eating certain plastics.

Polypropylene is also closed-loop recyclable[iv], meaning the recycled plastic can be used for the same types of products (including mushroom grow bags) as the virgin plastic. That is not true of all plastic types. Growers concerned about the issue should research the environmental impacts of several different grow set-ups to determine which is best in their situation.

[i] Mushroom Grow bags: The Ultimate Guide, accessed on 10 September, 2019.

[ii] The Permies website, accessed on 10 September, 2019.

[iii] Bradley, K. (2015). Mushroom Cultivation: Making Grain Spawn. Milkwood. Nov. 23, 2015.

[iv] LeBlanc, R. (2019). An Overview of Polypropylene Recycling. The Balance Small Business. May 9, 2015.

The Ultimate Guide to Mushroom Grow Bags for Fruiting and Spawn (2024)

FAQs

How to start fruiting mushroom bags? ›

You can trigger your mushrooms into fruiting by increasing humidity. Cut a cross into the front of your bag with a pair of scissors. You will need to pull down the flaps where you cut and sprinkle the mycelium with water several times per day.

When to fruit all in one grow bag? ›

Once colonization is complete, it's time for fruiting. To encourage fruiting in your all in one grow bag, cut or puncture holes in the bag for fresh air exchange. Place it in a bright location with high humidity. The mushrooms will then begin to form and mature within a few weeks.

What size bag for mushroom spawn? ›

Bag specifications:

Size 1: 100mm wide x 500mm tall with 40mm gussets - 0.2 micron filter patch. Size 2: 130mm wide x 500mm tall with 60mm gussets - 0.2 micron filter patch.

Where is the best place to put a mushroom grow bag? ›

While this can be good, airflow is also important and small enclosed spaces can cause your mushrooms to suffer from lack of oxygen. We recommend placing your grow kit on your kitchen bench, out of direct sunlight.

Should I shake my mushroom grow bag? ›

If the culture doesn't look uniform you may shake it for a few seconds to disperse the mycelium or spores.

How do you encourage mushroom fruiting? ›

To initiate fruiting, lowering temperatures and increasing oxygen levels generally encourages mushroom formation. Initiation can be in the form of cold shocking, water shocking or introducing more oxygen by cutting a hole in the bag.

When to cut open all in one mushroom bag? ›

Once your mushrooms have fully colonized the bag, you can either cut the holes or “x's” in the side of the bag, or cut the top of the bag off entirely in order to fruit your mushrooms. Cutting the bag with the edges at different heights will allow you some fine control over the humidity on the grow block.

How do I know when mycelium is fully colonized? ›

You can tell when mycelium is fully colonized when the entire substrate (such as a jar or a bag) is covered with a dense, white, and fuzzy network of mycelial threads. There should be no visible uncolonized areas or contaminants, and the mycelium should appear healthy and vigorous.

How long do you colonize all in one grow bags? ›

Colonization occurs after inoculation. This is when the mycelium will start to take over the grain that was inoculated with spores. This period usually lasts between 3 to 6 weeks depending on strain and environmental conditions. Ideal conditions for this phase are in a dark place and temperature between 72 F – 80 F.

What is the best substrate for mushroom grow bags? ›

Hardwoods such as oak, beech and maple make for a great substrate for many types of mushrooms, especially when combined with a bran supplement. Soy hulls can be mixed with hardwood sawdust at different ratios to produce extremely effective and high yielding substrates, particularly for growing oysters.

How long can you keep mycelium in spawn bag? ›

Grain spawn will store for three months in a refrigerator. The fresher the better, but mycelium is pretty resilient. If you don't get around to inoculating right away, tuck it away in your refrigerator until you're ready.

Do you water mushroom grow bags? ›

You don't need to water mushrooms like a plant. You don't need to water mushrooms like a plant. The spray bottle is for creating humidity. Humidity is a key factor for successful mushroom growth.

When to mix all in one mushroom grow bag? ›

Once your grain is between 50-80% colonized (white) break up the colonized grain thoroughly, and begin mixing into the substrate layer. It may help to lay the bag flat on its side and roll the material to mix.

How to tell when a spawn bag is ready? ›

begins, you should see white growth within 5-7 days and should quickly spread and fully colonize in anywhere from 14-21 days. After the jar is fully colonized, its best to wait and extra 2-3 days to make sure the center is also finished.

How to care for mushroom fruiting bags? ›

Simply cut the top off, keep moist and watch your mushrooms grow. We recommend you spray your mushrooms with water a couple of times a day. Oyster mushrooms are very resilient and will grow almost anywhere.

How do you activate a mushroom kit? ›

Original Mushroom Kit Instructions
  1. Open the box. If the compost looks brown, close the kit and leave it for a week. ...
  2. Open the plastic and evenly spread the casing over the compost, leaving it loose and fluffy. Leave the kit open.
  3. Every 2-3 days mist with water. After two to three weeks mushrooms will begin to form.

How long does it take for mycelium to start fruiting? ›

At this point in the growing process the mushroom mycelium should be pretty well established, and not that vulnerable to contaminates, but mold can and will grow in the terrarium if not well maintained. After 14 to 16 days, you should notice the beginnings of fruit bodies growing.

How long does it take for a spawn bag to colonize? ›

not see any progress right away but within 5-8 days you should see new growth all over the bag. This will continue until the bag is 100% colonized! ⇒ Complete colonization may take 30-45 days depending on the species and what temperature you incubate at.

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